HIV-1 Gag P15 Protéine

N° du produit ABIN2452192

Protéine Recombinant exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 3+ publications.

Aperçu rapide pour HIV-1 Gag P15 Protéine (ABIN2452192)

Antigène: HIV-1 Gag P15

Type de proteíne: Recombinant

Origine: Human Immunodeficiency Virus (HIV)

Source: Escherichia coli (E. coli)

Application: Dot Blot (DB), SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Pureté: > 90 % by SDS-PAGE (CBB staining)

Détail du produit

Information d'application

Indications d'application: 1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.42 mg/mL

Buffer: 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol

Stock: -20 °C

Référence

Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." dans: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." dans: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." dans: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Détail du antigène

Antigène: HIV-1 Gag P15

Classe de substances: Viral Protein

Sujet: HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA. HIV-1 Gag p15 was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B, and highly purified by several steps of chromatography. Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.